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Ilias Kounatidis et al.
G3 (Bethesda, Md.), 8(5), 1637-1647 (2018-03-15)
Candida glabrata (C. glabrata) forms part of the normal human gut microbiota but can cause life-threatening invasive infections in immune-compromised individuals. C. glabrata displays high resistance to common azole antifungals, which necessitates new treatments. In this investigation, we identified five
Ingrid A Edwards et al.
ACS infectious diseases, 3(12), 917-926 (2017-09-30)
Tachyplesin-1 (TP1; 1) is a cationic β-hairpin antimicrobial peptide with a membranolytic mechanism of action. While it possesses broad-spectrum, potent antimicrobial activity, 1 is highly hemolytic against mammalian erythrocytes, which precludes it from further development. In this study, we report
Alysha G Elliott et al.
Nature communications, 11(1), 3184-3184 (2020-06-25)
Peptide antibiotics are an abundant and synthetically tractable source of molecular diversity, but they are often cationic and can be cytotoxic, nephrotoxic and/or ototoxic, which has limited their clinical development. Here we report structure-guided optimization of an amphipathic peptide, arenicin-3
Xiao Liu et al.
Materials (Basel, Switzerland), 13(16) (2020-08-14)
Ammonia nitrogen and natural organic matter (NOM) seriously degrade the quality of surface waters. In this study, the optimum preparation conditions of a yeast-chitosan bio-microcapsule of the Candida tropicalis strain, used to treat micro-polluted surface water, were investigated. Fourier transform
Michal Bittner et al.
Chemosphere, 132, 56-62 (2015-03-24)
Recombinant yeast assays (RYAs) constitute a suitable tool for the environmental monitoring of compounds with endocrine disrupting activities, notably estrogenicity and androgenicity. Conventional procedures require yeast reconstitution from frozen stock, which usually takes several days and demands additional equipment. With
William T Kay et al.
Fungal genetics and biology : FG & B, 128, 14-19 (2019-03-17)
Zymoseptoria tritici, the causal agent of Septoria tritici blotch, is a notable pathogen of temperate-grown wheat. To better understand the mechanisms underpinning pathogenicity, leaf infection assays are commonly used to compare either the virulence of Z. tritici wildtype or mutant
Difco Manual, 571-571 (1998)
Anne Kerlikowski et al.
Journal of biophotonics, 13(12), e202000221-e202000221 (2020-09-16)
Reinfection in endodontically treated teeth is linked to the complexity of the root canal system, which is problematic to reach with conventional disinfection methods. As plasma is expected to have the ability to sanitize narrow areas, the aim of this
Sergio R Santa Maria et al.
Astrobiology (2020-01-07)
The biological risks of the deep space environment must be elucidated to enable a new era of human exploration and scientific discovery beyond low earth orbit (LEO). There is a paucity of deep space biological missions that will inform us
Thomas S Dash et al.
Structure (London, England : 1993), 27(2), 315-326 (2018-12-18)
Disulfide-rich peptides (DRPs) play diverse physiological roles and have emerged as attractive sources of pharmacological tools and drug leads. Here we describe the 3D structure of a centipede venom peptide, U-SLPTX15-Sm2a, whose family defines a unique class of one of
D A Treco et al.
Current protocols in molecular biology, Chapter 13, Unit13-Unit13 (2008-02-12)
Preparation of sterile media of consistently high quality is essential for the genetic manipulation of yeast. Recipes for media needed in the protocols in this chapter are provided in this unit. Specific suppliers are recommended for specific ingredients.
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