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Fatemeh Kiaeian Moosavi et al.
Archives of insect biochemistry and physiology, 103(4), e21655-e21655 (2020-01-19)
Some studies have highlighted benefits for Lobesia botrana by adding Botrytis cinerea mycelium to an artificial larval diet and have suggested a mutualistic relationship between the two organisms on grapevine, hypothesizing that fungal sterols were the nutritional factor involved. Because
Melanie Hornschuh et al.
Acta biomaterialia, 106, 376-386 (2020-02-19)
Immobilized polycationic substances on biomaterial surfaces kill adhering bacteria upon contact and are considered a promising non-antibiotic alternative. Unfortunately, there is no generally accepted in vitro method for quantitatively evaluating the antibacterial efficacy of contact-active non-leachable antimicrobial surfaces. Moreover, guidelines
Antonella Denise Losinno et al.
Autophagy, 17(2), 439-456 (2020-01-28)
Cruzipain, the major cysteine protease of the pathogenic protozoa Trypanosoma cruzi, is an important virulence factor that plays a key role in the parasite nutrition, differentiation and host cell infection. Cruzipain is synthesized as a zymogen, matured, and delivered to
A Adekunte et al.
International journal of food microbiology, 137(1), 116-120 (2009-11-13)
Power ultrasound is recognised as a potential non thermal technique to inactivate microorganisms pertinent to fruit juices. In this study, the effect of sonication on the resistance of yeast (Pichia fermentans) in tomato juice was investigated. Tomato juice samples were
Alka Srivastava et al.
Bio-protocol, 13(10), e4677-e4677 (2023-06-01)
Cotton is a significant industrial crop, playing an essential role in the global economy that suffers several setbacks due to biotic and abiotic adversities. Despite such problems, biotechnological advances in cotton are limited because of genetic transformation and regeneration limitations.
Jinyou Zhang et al.
Biotechnology and bioengineering, 82(6), 640-652 (2003-04-04)
Yeast extract (YE) is commonly used as a key component in the complex media for industrial fermentations. However, the lot-to-lot variation of this raw material frequently requires extensive "use testing" of many lots to identify only the few that support
C Graham Clark et al.
Current protocols in microbiology, 43, 20A-20A (2016-11-20)
Blastocystis is an intestinal parasite that is very easily isolated in culture from fresh stool samples. In fact, the parasite grows so readily in culture that short-term in vitro culture is sometimes used as a diagnostic tool in the absence
Juan Andres Imelio et al.
Bio-protocol, 7(16), e2510-e2510 (2017-08-20)
We have developed protocols to generate site-specific variants of the histidine-kinase DesK and its cognate response regulator DesR, conducive to trapping different signaling states of the proteins. Co-expression of both partners in E. coli, ensuring an excess of the regulator
Jing Li et al.
Bio-protocol, 7(15), e2434-e2434 (2017-08-05)
Streptococcus pneumoniae (pneumococcus) is an important human pathogen that causes pneumonia, meningitis, sepsis, and otitis media. This bacterium normally resides in the nasopharynx as a commensal, but sometimes disseminates to sterile sites of humans and causes local or systemic inflammation.
Kuo-Fu Tseng et al.
Current biology : CB, 30(18), 3664-3671 (2020-08-01)
Kinesin-14s are microtubule-based motor proteins that play important roles in mitotic spindle assembly [1]. Ncd-type kinesin-14s are a subset of kinesin-14 motors that exist as homodimers with an N-terminal microtubule-binding tail, a coiled-coil central stalk (central stalk), a neck, and
João Pedro Amorim et al.
STAR protocols, 1(3), 100208-100208 (2020-12-31)
Since its first application for site-directed mutagenesis, the CRISPR-Cas9 system has revolutionized genome engineering. Here, we present a validated workflow for the generation of targeted genomic deletions in zebrafish, including the design, cloning, and synthesis of single-guide RNAs and Cas9
Olena P Ishchuk et al.
Antibiotics (Basel, Switzerland), 9(1) (2020-01-08)
The opportunistic human fungal pathogen Candida albicans relies on cell morphological transitions to develop biofilm and invade the host. In the current study, we developed new regulatory molecules, which inhibit the morphological transition of C. albicans from yeast-form cells to
Omid Zarei et al.
Iranian journal of pharmaceutical research : IJPR, 15(4), 907-913 (2017-03-01)
Yeasts, especially Saccharomyces cerevisiae, are one of the oldest organisms with broad spectrum of applications, owing to their unique genetics and physiology. Yeast extract, i.e. the product of yeast cells, is extensively used as nutritional resource in bacterial culture media.
Rational Discovery of (+) (S) Abscisic Acid as a Potential Antifungal Agent: a Repurposing Approach.
Mohammed A Khedr et al.
Scientific reports, 8(1), 8565-8565 (2018-06-06)
Fungal infections are spreading widely worldwide, and the types of treatment are limited due to the lack of diverse therapeutic agents and their associated side effects and toxicity. The discovery of new antifungal classes is vital and critical. We discovered
Sara Hooshangi et al.
PLoS computational biology, 7(9), e1002172-e1002172 (2011-10-08)
Quorum sensing (QS) enables bacterial multicellularity and selective advantage for communicating populations. While genetic "switching" phenomena are a common feature, their mechanistic underpinnings have remained elusive. The interplay between circuit components and their regulation are intertwined and embedded. Observable phenotypes
Pascal Chatonnet et al.
Journal of agricultural and food chemistry, 58(23), 12481-12490 (2010-11-10)
This study identifies a previously isolated bacterium as Rhizobium excellensis, a new species of proteobacteria able to form a large quantity of 2-methoxy-3,5-dimethylpyrazine (MDMP). R. excellensis actively synthesizes MDMP from L-alanine and L-leucine and, to a lesser extent, from L-phenylalanine
Benjamin A Adducci et al.
Biosensors & bioelectronics, 78, 160-166 (2015-11-26)
New methods and technology are needed to quickly and accurately detect potential biological warfare agents, such as Bacillus anthracis, causal agent of anthrax in humans and animals. Here, we report the detection of a simulant of B. anthracis (B. globigii)
Lucija Markulin et al.
Planta, 250(1), 347-366 (2019-05-01)
The involvement of a WRKY transcription factor in the regulation of lignan biosynthesis in flax using a hairy root system is described. Secoisolariciresinol is the main flax lignan synthesized by action of LuPLR1 (pinoresinol-lariciresinol reductase 1). LuPLR1 gene promoter deletion
Lukas Schertel et al.
Journal of the Royal Society, Interface, 17(166), 20200196-20200196 (2020-05-21)
Vivid colours found in living organisms are often the result of scattering from hierarchical nanostructures, where the interplay between order and disorder in their packing defines visual appearance. In the case of Flavobacterium IR1, the complex arrangement of the cells
Colin Charnock
Microorganisms, 9(1) (2021-01-07)
Plastic pollution has become one of the most critical environmental issues, as rapidly increasing production, compounded by persistence of plastic wastes in the environment, are outpacing efforts to keep ecosystems plastic-free. A switch to plastics more amenable to microbial attack
Daeun Moon et al.
Annals of clinical and laboratory science, 50(1), 31-44 (2020-03-13)
The microbiome has recently become a key interest for cancer research. Anti-tumor effects of reinforced clostridium media (RCM) were investigated for all ingredients of RCM, which showed that yeast extract could be a candidate for this phenomenon. MTT assay, cell
Marco R Binelli et al.
Biomaterials advances, 141, 213095-213095 (2022-09-06)
Bacterial cellulose is an attractive resource for the manufacturing of sustainable materials, but it is usually challenging to shape it into elaborate three-dimensional structures. Here, we report a manufacturing platform for the creation of complex-shaped cellulose objects by printing inks
Daeun Moon et al.
Molecular medicine reports, 20(4), 3933-3941 (2019-08-23)
The microbiome has recently attracted research interest in a variety of subjects, including cancer. In the present study, it was determined that reinforced clostridium media (RCM) for microbiome culture, exerts antitumor effects on renal cell carcinoma cells when compared to
Cecilia Arahí Prato et al.
STAR protocols, 1(3), 100204-100204 (2020-12-31)
Galectins are soluble lectins that participate in many physiological and pathological functions. Since they can act extracellularly, the use of the recombinant protein is a recurrent strategy for studying their biological functions. Here, we provide a general protocol for the
Losee L Ling et al.
Nature, 517(7535), 455-459 (2015-01-07)
Antibiotic resistance is spreading faster than the introduction of new compounds into clinical practice, causing a public health crisis. Most antibiotics were produced by screening soil microorganisms, but this limited resource of cultivable bacteria was overmined by the 1960s. Synthetic
Carolina Fernandez-Senac et al.
Journal of fish diseases, 43(11), 1463-1472 (2020-09-04)
Routine gill swabbing is a non-destructive sampling method used for the downstream qPCR detection and quantitation of the pathogen Neoparamoeba perurans, a causative agent of amoebic gill disease (AGD). Three commercially available swabs were compared aiming their application for timelier
Joshua E Mayfield et al.
Bio-protocol, 10(12), e3648-e3648 (2021-03-05)
Eukaryotic RNA polymerase II transcribes all protein-coding mRNAs and is highly regulated. A key mechanism directing RNA polymerase II and facilitating the co-transcriptional processing of mRNAs is the phosphorylation of its highly repetitive carboxyl-terminal domain (CTD) of its largest subunit
Cynthia Vanesa Rivero et al.
Frontiers in cellular and infection microbiology, 11, 657257-657257 (2021-09-04)
T. cruzi, the causal agent of Chagas disease, is a parasite able to infect different types of host cells and to persist chronically in the tissues of human and animal hosts. These qualities and the lack of an effective treatment
Samriddha Ray et al.
The Journal of cell biology, 190(5), 793-805 (2010-09-02)
The mechanisms that regulate cytoskeletal remodeling during the transition between mitosis and interphase are poorly understood. In fission yeast the MOR pathway promotes actin polarization to cell tips in interphase, whereas the SIN signaling pathway drives actomyosin ring assembly and
Luciana M Katiki et al.
Veterinary parasitology, 182(2-4), 264-268 (2011-06-18)
The most challenging obstacles to testing products for their anthelmintic activity are: (1) establishing a suitable nematode in vitro assay that can evaluate potential product use against a parasitic nematode of interest and (2) preparation of extracts that can be
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