技术规格说明书
产品名称
GenElute™-E Single Spin Cell Culture DNA Kit Reagents and consumables for various cell culture sample types. 10, 50 or 250 purifications.
产品编号
ec400
产品品牌
sigma
化学文摘社编号
分子量
Test皿 | 规格 |
|---|---|
| Expiry | Report result |
| Yield/mg tissue (µg/mg) | > 2 µg DNA / mg tissue |
| Concentration (ng/µl) | ≥ 25 ng/µL |
| PCR functionality | ≤ 28 Ct |
| Ratio 260/280 | 1.4-2.1 |
| Eluate volume | 80-105 µL |
| Eluate OD 230 | < 0.2 |
| Appearance in Agarose Gel | Genomic DNA band clearly visible |
| Clogging of column | No clogging observed |
| Colour-free eluate | No reddish colour in eluate |
| Spin Columns (Lot 9004) | Pass |
| Void OD A230 | 0.1 |
| Eluate OD A230 | 0.07 mL |
| Resin concentration (mL) | 6.75 mL |
| Salt depletion / conductivity [% or µS/cm] | 96% /< 200 µS/cm |
| PCR (FAM) | Ct 29 |
| PCR (VIC) | Ct 29 |
| DNA recovery rate (%) | 0.763 |
| Agarose gel | Depletion visible maximum in 300bp band |
| 1x Tris Buffer (8980) | Pass |
| pH target value | pH 8.5 |