技术规格说明书
产品名称
KiCqStart® Probe qPCR ReadyMix™，低ROX™ with Low ROX for ABI and Stratagene instruments
产品编号
kcqs05
产品品牌
sigma
化学文摘社编号
分子量
Test皿 | 规格 |
|---|---|
| 1-Step RT-qPCR Functional Assay | Fast-cycling Real-time PCR detection of log-fold serial dilutions of a control DNA from 107 to 10 copies. Linear regression analysis of cycle threshold versus log input quantity must give a slope of between –3.2 and –3.6 and coefficient of determination (r2) ≥0.990 |
| RT-qPCR genomic DNA Functional Assay | Real-time PCR detection of single-copy gene in human genomic DNA using activation step of 10 minutes at 95°C. Linear regression analysis of cycle threshold versus log input quantity for a log-fold serial dilutions of human genomic DNA from 105 to 10 copies must give a slope of between –3.2 and –3.6 and coefficient of determination (r2) ≥0.990 with accurate two-fold discrimination of 500, 1000, and 2000 copies. |
| DNase | DNase activity must be below the detectable limits of 100 pg DNase I equivalent as assayed using a fluorogenic substrate following a 1 hour incubation at 37°C with each kit component at 1X concentration. |
| Rnase | RNase activity must be below the detectable limits of 1 pg RNase A equivalent as assayed using a fluorogenic substrate following a 1 hour incubation at 37°C with each kit component at 1X concentration. |
| Expiration Date | Report result |