A comparative study of the analysis of human urine headspace using gas chromatography-mass spectrometry.

A comparative study of the analysis of human urine headspace using gas chromatography-mass spectrometry.

Journal of breath research (2008-09-01)

S Smith, H Burden, R Persad, K Whittington, B de Lacy Costello, N M Ratcliffe, C S Probert

摘要

First-void urine samples were obtained from 24 elderly, asymptomatic men (median age 62.9 years). The headspace above pH adjusted urine samples were extracted using a carboxen/polydimethylsiloxane solid phase micro-extraction fibre and the volatile organic compounds analysed by gas chromatography/mass spectrometry. A total of 147 compounds were identified in the headspace of urine. The acidified samples recorded a total of 92 compounds, 27 of which were ubiquitous, basified samples 70 compounds, with 12 ubiquitous and unmodified pH samples 49, with 6 ubiquitous. Five compounds were ubiquitous irrespective of pH: acetone, methylene chloride, 4-heptanone, 2-pentanone and 2-butanone. A comparative analysis of unfrozen and frozen-thawed urine (stored at room temperature for 0, 1 and 8 h) showed that samples retained the same number of compounds although variations in the peak areas for some compounds were observed.

材料

产品编号

品牌

产品描述

57298-U

Supelco

SPME萃取头组件, 50/30µm DVB/CAR/PDMS, StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 3, plain gray hub

57348-U

Supelco

SPME萃取头组件, 50/30µm DVB/CAR/PDMS, StableFlex (2cm), needle size 24 ga, Autosampler, pk of 3, notched gray hub

57328-U

Supelco

SPME萃取头组件, 50/30µm DVB/CAR/PDMS, StableFlex (1cm), needle size 24 ga, Manual Holder, pk of 3, plain gray hub

57299-U

Supelco

SPME萃取头组件, 50/30µm DVB/CAR/PDMS, StableFlex (2cm), needle size 23 ga, Autosampler, pk of 3, notched gray hub

57341-U

Supelco

SPME 纤维组件聚二甲基硅氧烷 (PDMS), 100 μm PDMS, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 3, red hub

57329-U

Supelco

SPME萃取头组件, 50/30µm DVB/CAR/PDMS, StableFlex (1 cm), needle size 24 ga, Autosampler, pk of 3, plain gray hub

20358

Supelco

ORBO^™ Carboxen-564 管, W,F,F separators, O.D. × L 6 mm × 75 mm, pkg of 25 ea, for analyte group MEK (methylethyl ketone)

20355

Supelco

ORBO-78 400/200mg，每包 25 支, W,W,W separators, O.D. × L 6 mm × 110 mm, pkg of 25 ea

25467

Supelco

Carboxen^®1010 多孔层壁涂开管柱, L × I.D. 30 m × 0.53 mm, average thickness 30 μm

57300-U

Supelco

SPME 纤维组件聚二甲基硅氧烷 (PDMS), 100 μm PDMS, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3, red hub

10478-U

Supelco

Carbopack^®吸附剂, matrix Carboxen^® 1000, 60-80 mesh, bottle of 10 g

57318

Supelco

SPME固相微萃取萃取头 Carboxen/聚二甲基硅氧烷 (CAR/PDMS), 75 μm CAR/PDMS, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3, black hub

57301

Supelco

SPME 纤维组件聚二甲基硅氧烷 (PDMS), 100 μm PDMS, Fused Silica (1 cm), needle size 24 ga, Autosampler, pk of 3, red hub

57310-U

Supelco

SPME固相微萃取萃取头，二甲基硅氧烷/二乙烯基苯(PDMS/DVB), 65 μm PDMS/DVB, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3, blue hub

25461

Supelco

Carboxen^® 1006 多孔层壁涂开管柱, L × I.D. 30 m × 0.53 mm, average thickness 30 μm

57319

Supelco

SPME固相微萃取萃取头 Carboxen/聚二甲基硅氧烷 (CAR/PDMS), 75 μm CAR/PDMS, Fused Silica (1 cm), needle size 24 ga, Autosampler, pk of 3, black hub

57295-U

Supelco

SPME固相微萃取萃取头 Carboxen/聚二甲基硅氧烷 (CAR/PDMS), 85 μm CAR/PDMS, StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 3, light blue hub

57293-U

Supelco

SPME固相微萃取萃取头，二甲基硅氧烷/二乙烯基苯(PDMS/DVB), 65 μm PDMS/DVB, StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 3, pink hub

57311

Supelco

SPME固相微萃取萃取头，二甲基硅氧烷/二乙烯基苯(PDMS/DVB), 65 μm PDMS/DVB, Fused Silica (1 cm), needle size 24 ga, Autosampler, pk of 3, blue hub

57326-U

Supelco

SPME固相微萃取萃取头，二甲基硅氧烷/二乙烯基苯(PDMS/DVB), 65 μm PDMS/DVB, StableFlex (1 cm), needle size 24 ga, Manual Holder, pk of 3, pink hub

24246

Supelco

Carboxen^®1010 多孔层壁涂开管柱, L × I.D. 30 m × 0.32 mm, average thickness 15 μm

57334-U

Supelco

SPME固相微萃取萃取头 Carboxen/聚二甲基硅氧烷 (CAR/PDMS), 85 μm CAR/PDMS, StableFlex (1 cm), needle size 24 ga, Manual Holder, pk of 3, light blue hub

57305

Supelco

聚丙烯酸酯 (PA) 固相微萃取萃取头, 85 μm Polyacrylate, Fused Silica (1 cm), needle size 24 ga, Autosampler, pk of 3, white hub

57327-U

Supelco

SPME固相微萃取萃取头，二甲基硅氧烷/二乙烯基苯(PDMS/DVB), 65 μm PDMS/DVB, StableFlex (1 cm), needle size 24 ga, Autosampler, pk of 3, pink hub

57359-U

Supelco

固相微萃取便携式现场采样器, coating PDMS/DVB

57335-U

Supelco

SPME固相微萃取萃取头 Carboxen/聚二甲基硅氧烷 (CAR/PDMS), 85 μm CAR/PDMS, StableFlex (1 cm), needle size 24 ga, Autosampler, pk of 3, light blue hub

57346-U

Supelco

SPME固相微萃取萃取头，二甲基硅氧烷/二乙烯基苯(PDMS/DVB), 65 μm PDMS/DVB, Fused Silica (1 cm), needle size 23 ga, Manual Holder, pk of 3, blue hub

57294-U

Supelco

聚丙烯酸酯 (PA) 固相微萃取萃取头, 85 μm Polyacrylate, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 3, white hub

504823

Supelco

固相微萃取便携式现场采样器, coating PDMS

23446-U

Supelco

ORBO^™-93吸附管, ORBO tube I.D. × L 6 mm × 95 mm, Bed A 180 mg, Bed B 90 mg, 60/80 mesh, pkg of 25 ea