Activation of the MAP kinase homologue RK requires the phosphorylation of Thr-180 and Tyr-182 and both residues are phosphorylated in chemically stressed KB cells.

Activation of the MAP kinase homologue RK requires the phosphorylation of Thr-180 and Tyr-182 and both residues are phosphorylated in chemically stressed KB cells.

FEBS letters (1995-05-08)

Y N Doza, A Cuenda, G M Thomas, P Cohen, A R Nebreda

摘要

A MAP kinase homologue, termed the reactivating kinase (RK), lies in a signalling pathway which mediates cellular responses to stress. Here we demonstrate that the stress-induced activation of the RK in human KB cells is accompanied by the phosphorylation of Thr-180 and Tyr-182, and that the phosphorylation of both residues is required for the activation of this enzyme.

材料

货号

品牌

产品描述

14-251-M

Sigma-Aldrich

p38α/SAPK2a Protein, active, 10 µg, Active, N-terminal GST tagged, recombinant, full length p38α/SAPK2a, for use in Kinase Assays.