- Detection of exosomal prions in blood by immunochemistry techniques.
Detection of exosomal prions in blood by immunochemistry techniques.
The Journal of general virology (2015-03-26)
Francesca Properzi, Mariantonia Logozzi, Hanin Abdel-Haq, Cristina Federici, Luana Lugini, Tommaso Azzarito, Ilaria Cristofaro, Daniela di Sevo, Elena Ferroni, Franco Cardone, Massimo Venditti, Marisa Colone, Emmanuel Comoy, Valérie Durand, Stefano Fais, Maurizio Pocchiari
PMID25805411
摘要
In most forms of prion diseases, blood is infectious, but detection by immunochemistry techniques of the only available marker of infection (the misfolded prion protein, PrPTSE) in blood remains elusive. We developed a novel method for the detection of PrPTSE in blood of prion-infected rodents based on the finding that PrPTSE is associated with plasma exosomes. However, further purification of the exosomes on a sucrose gradient was necessary to remove plasma immunoglobulins, which interfere with PrPTSE, masking its detection by immunochemistry. Finally, we report that about 20% of plasma infectivity is associated with exosomes.
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