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Merck
CN

p38 Expression and Modulation of STAT3 Signaling in Oral Cancer.

Pathology oncology research : POR (2018-03-23)
I Gkouveris, N Nikitakis, A Sklavounou
摘要

p38 protein belongs to Mitogen-activated protein kinases family that link extracellular stimuli with intracellular responses participating in numerous of fundamental cell processes. Persistent activation of STAT3 has been associated with cell proliferation, differentiation and apoptosis in oral squamous cell carcinoma (OSCC). This study examines the effects of p38 modulation on STAT3 signaling and cellular activities in OSCC cells and investigates possible correlation of p38 expression with tumor degree of differentiation. Phospho-p38 immunostaining was performed in 60 OSCC including well, moderately and poorly differentiated tumors. Semiquantitative analysis was used, by calculating intensity, percentage and combined scores. Protein expression levels of STAT3 (total, tyrosine and serine phosphorylated), p38 and cyclin D1 were assessed in two OSCC cell lines. p38 inhibition was achieved by pharmacological agent(SB2023580). Cell proliferation and viability rates were also evaluated. Phospho-p38 immunoexpression was intense in almost all tumor specimens, nevertheless did not correlate with tumor differentiation. Inhibition of p38 with SB203580 did not appear to affect tyrosine or serine phosphorylated STAT3 as well as cyclin D1 levels in both cell lines. Moreover, p38 inhibition resulted in mild dose-dependent decreases in cell growth and viability in both cell lines. p38 is highly expressed in OSCC but does not seem to mediate the oncogenic STAT3 pathway. However, changes found in proliferation and viability may suggest that p38 functions as potent regulator of HNSCC. Understanding the complexity of p38 signaling and cross-talk between other major molecules, may guide the development of novel pharmacologic therapies for cancer treatment and prevention.

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杜氏改良 Eagle 培养基 - 高葡萄糖, With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture
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柠檬酸盐缓冲液,0.09M
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25 mg
预计发货时间 2025年7月07日
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¥754.26
100 mg
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¥931.39
500 mg
预计发货时间 2025年7月07日
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¥3,615.37
1 g
预计发货时间 2025年7月07日
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¥6,827.00
10 g
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SAFC
Ham 营养混合物 F12, liquid
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货号包装规格是否有货价格数量
25 mg
预计发货时间 2025年7月07日
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¥754.26
100 mg
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¥931.39
500 mg
预计发货时间 2025年7月07日
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¥3,615.37
1 g
预计发货时间 2025年7月07日
详情...
¥6,827.00
10 g
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¥30,101.96
25 g
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¥60,075.83