Rapid and easy multiresidue method for the analysis of antibiotics in meats by ultrahigh-performance liquid chromatography-tandem mass spectrometry.

Rapid and easy multiresidue method for the analysis of antibiotics in meats by ultrahigh-performance liquid chromatography-tandem mass spectrometry.

Journal of agricultural and food chemistry (2015-02-07)

Takahiro Yamaguchi, Masahiro Okihashi, Kazuo Harada, Kotaro Uchida, Yoshimasa Konishi, Keiji Kajimura, Kazumasa Hirata, Yoshimasa Yamamoto

PMID25656008

摘要

This study involved the development of a multiresidue method for the rapid analysis of 43 antibiotics in meats using ultrahigh-performance liquid chromatography-tandem mass spectrometry. This method was performed using dispersive-solid phase extraction, which is able to analyze 20 samples within 2 h. All compounds were determined simultaneously on a C18 separation column with gradient elution. Validation of the analytical method was performed by carrying out linearity, limit of quantification (LOQ), accuracy, precision, and recovery tests in different meat products. The validation criteria were set according to AOAC International and Japanese validation guidelines. The linearity of each compound was almost the coefficient of determination (r(2)) > 0.98. The LOQs of all tested antibiotics were <10 μg/kg. The results verify that this method is capable of quantitative analysis of 36, 33, and 37 compounds in beef, pork, and chicken, respectively. This method can be used for rapid and easy multiresidue screening of antibiotics for three meats (pork, beef, and chicken).

材料

货号

品牌

产品描述

ZIX7010P0C

Milli-Q^® IX Pure Water System with E-POD^® Dispenser, output: type 2 water (> 5 MΩ·cm), input: potable tap water, The most advanced pure water system for the production of Elix^{^®} quality water at a flow rate of 10 L/h, with E-POD^® pure water dispenser.

ZIX7005P0C

Milli-Q^® IX Pure Water System with E-POD^® Dispenser, output: type 2 water (> 5 MΩ·cm), input: potable tap water, The most advanced pure water system for the production of Elix^{^®}quality water at a flow rate of 5 L/h, with E-POD^® pure water dispenser.

ZIX7015P0C

Milli-Q^® IX Pure Water System with E-POD^® Dispenser, input: potable tap water, output: type 2 water (> 5 MΩ·cm), The most advanced pure water system for the production of Elix^{^®}quality water at a flow rate of 15 L/h, with E-POD^® pure water dispenser.

ZIX7005T0C

Milli-Q

Milli-Q^® IX 7005净水系统, input: potable tap water, output: type 2 water (> 5 MΩ·cm), The most advanced pure water system for the production of Elix^® quality water at a flow rate of 5 L/h.

ZIX7003T0C

Milli-Q

Milli-Q^® IX 7003净水系统, input: potable tap water, output: type 2 water (> 5 MΩ·cm), The most advanced pure water system for the production of Elix^® quality water at a flow rate of 3 L/h.

ZIX7010T0C

Milli-Q

Milli-Q^® IX 7010净水系统, input: potable tap water, output: type 2 water (> 5 MΩ·cm), The most advanced pure water system for the production of Elix^® quality water at a flow rate of 10 L/h.

ZIX7015T0C

Milli-Q

Milli-Q^® IX 7015净水系统, output: type 2 water (> 5 MΩ·cm), input: potable tap water, The most advanced pure water system for the production of Elix^® quality water at a flow rate of 15 L/h.

ZIX7003P0C

Milli-Q^® IX Pure Water System with E-POD^® Dispenser, output: type 2 water (> 5 MΩ·cm), input: potable tap water, The most advanced pure water system for the production of Elix^® quality water at a flow rate of 3 L/h, with E-POD^® pure water dispenser.