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Merck
CN

A restriction endonuclease from Staphylococcus aureus.

Nucleic acids research (1976-11-01)
J S Sussenbach, C H Monfoort, R Schiphof, E E Stobberingh
摘要

A specific endonuclease, Sau 3AI, has been partially purified from Staphylococcus aureus strain 3A by DEAE-cellulose chromatography. The enzyme cleaves adenovirus type 5 DNA many times, SV40 DNA eight times but does not cleave double-stranded phi X174 DNA. It recognizes the sequence (see article) and cleaves as indicated by the arrows. Evidence is presented that this enzyme plays a role in the biological restriction-modification system of Staphylococcus aureus strain 3A.