Cellulose nanocrystals incorporated β-chitosan nanoparticles to enhance the stability and in vitro release of β-galactosidase.

Beta-galactosidase (β-gal), catalyzing the transformation of lactose to glucose and galactose, had been encapsulated in β-chitosan nanoparticles (β-CS NPs) in previous work, but they were prone to aggregation and disscociation, resulting in poor bioavailability of β-gal. Herein, we developed cellulose nanocrystals (CNC, as stabilizers and fillers) stabilized β-gal loaded low molecular weight (LMW) β-CS NPs through ionic gelation technology to enhance enzyme activity and further control in vitro release of β-gal. Results showed that particle size and Zeta potential (ZP) of CNCs stabilized β-gal loaded CS NPs were 143.20 nm and -34.70 mV under the optimal conditions, respectively. Structural analysis were employed to study the incorporation of β-gal and CNC into β-CS NPs. In vitro release study conducted at pH 4.5 and 7.4 showed that both β-gal loaded β-CS NPs and CNC stabilized ones retained the release of β-gal for over 12 h. Moreover, CNC stabilized β-gal loaded β-CS NPs retained higher β-gal activity (81.23%) than that of controls (30%) within 2 h. Therefore, it was indicated that CNC incorporated β-CS NPs could serve as non-toxic and effective carriers of β-gal for the treatment of lactose intolerance.