- Extraction of Soluble and Insoluble Protein Fractions from Mouse Brains and Spinal Cords.
Extraction of Soluble and Insoluble Protein Fractions from Mouse Brains and Spinal Cords.
Bio-protocol (2017-08-05)
Oliver Wirths
PMID34541150
摘要
The current protocol details the preparation of soluble and insoluble protein lysates from mouse brain or spinal cord samples. In detail, tissue homogenization and sequential protein extraction are described. This procedure yields soluble and insoluble protein extracts that can be further processed in down-stream applications like ELISA or Western blotting.
材料
产品编号
品牌
产品描述
Millipore
Benzonase®核酸酶, ≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution