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Merck
CN

A permeability assay for mouse intestinal organoids.

STAR protocols (2022-05-12)
Stijn Aaron den Daas, Ugo Soffientini, Shilpa Chokshi, Gautam Mehta
摘要

Here, we describe an assay for intestinal permeability in mouse intestinal organoids, although this may also be adapted for other species. Propidium iodide (PI) does not penetrate intact biological membranes and thus cannot enter the lumen of intact organoids. Passage of PI within the lumen can be induced by tight junction disruption or epithelial cell death. This technique measures PI-stained extruded dead cells within the organoid lumen to analyze the effect of insults, toxins, or treatments on intestinal organoid permeability.

材料
Product Number
品牌
产品描述

Sigma-Aldrich
N-乙酰基-L-半胱氨酸, BioReagent, suitable for cell culture
Sigma-Aldrich
乙二胺四乙酸 二钠盐 二水合物, reagent grade, 98.5-101.5% (titration)