- A new restriction endonuclease from Acetobacter pasteurianus.
A new restriction endonuclease from Acetobacter pasteurianus.
Nucleic acids research (1983-07-11)
J Seurinck, A Van de Voorde, M Van Montagu
PMID6306590
摘要
A restriction endonuclease, ApaI, has been partially purified from Acetobacter pasteurianus. This enzyme cleaves bacteriophage lambda DNA and Simian virus 40 DNA at one site, adenovirus-2 DNA at more than nine sites, but it does not cleave phi X174 DNA nor plasmid pBR322 DNA. This enzyme recognizes the sequence (formula; see text) and cuts at the sites indicated by the arrows.