Interaction of the restriction endonuclease ScaI with its substrates.

Interaction of the restriction endonuclease ScaI with its substrates.

Nucleic acids research (1985-10-11)

K Kita, N Hiraoka, F Kimizuka, A Obayashi, H Kojima, H Takahashi, H Saito

摘要

The kinetic constants of the site-specific endonuclease, ScaI, for various substrates were determined. We estimated Vmax and Km for octa-, deca-, dodeca-, and hexadecanucleotides and for plasmid pBR322 DNA. Vmax for these substrates were close, but Km were quite different (in decreasing order, octa- greater than deca-, dodeca-, hexadeca- greater than pBR322). The results were discussed with respect to the tertiary structure of substrate.