- On-chip electrochemical measurement of beta-galactosidase expression using a microbial chip.
On-chip electrochemical measurement of beta-galactosidase expression using a microbial chip.
Chemical communications (Cambridge, England) (2004-01-23)
Takatoshi Kaya, Kuniaki Nagamine, Nobuto Matsui, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue
PMID14737572
摘要
[small beta]-Galactosidase expression in a small number of Escherichia coli cells has been measured in real time with an electrochemical sensor chip. E. coli cells were embedded using collagen gel within a micropore which was microfabricated onto a chip. The activity of the expressed [small beta]-galactosidase was determined using p-aminophenyl [small beta]-d-galactopyranoside (PAPG) as a substrate.
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