Purification of lysyl oxidase from piglet skin by selective interaction with Sephacryl S-200.

Purification of lysyl oxidase from piglet skin by selective interaction with Sephacryl S-200.

The Biochemical journal (1990-03-15)

D R Shackleton, D J Hulmes

PMID1970238

摘要

A method is described for the purification of piglet skin lysyl oxidase from a crude urea extract by two simple chromatographic procedures. The method relies on the selective interaction between Sephacryl S-200 and lysyl oxidase that occurs in the absence of urea and in conditions of low ionic strength.

材料

产品编号

品牌

产品描述

S200HR

Sigma-Aldrich

Sephacryl^®, 200-HR, MW range 5-250 kDa (globular proteins), MW range 1-80 kDa (dextrans)

S300HR

Sigma-Aldrich

Sephacryl^®, 300-HR, MW range 10-1500 kDa (globular proteins), MW range 1-400 kDa (dextrans)

S400HR

Sigma-Aldrich

Sephacryl^®, 400-HR, MW range 20-8000 kDa (globular proteins)

S100HR

Sigma-Aldrich

Sephacryl^®, 100-HR, MW range 1000-100,000 Da (globular proteins)

S500HR

Sigma-Aldrich

Sephacryl^®, 500-HR, MW range 40-20,000 kDa (dextrans)