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Merck
CN

Analytical applications of enzymatic growth of quantum dots.

Chemistry (Weinheim an der Bergstrasse, Germany) (2010-05-01)
Laura Saa, Ana Virel, Jose Sanchez-Lopez, Valery Pavlov
摘要

We have developed an analytical assay to detect the enzymatic activity of acetylcholine esterase and alkaline phosphatase based on the generation of quantum dots by enzymatic products. Acetylcholine esterase converts acetylthiocholine into thiocholine. The latter enhances the rate of decomposition of sodium thiosulfate into H(2)S, which in the presence of cadmium sulfate yields CdS quantum dots showing a time dependent exponential growth, typical of autocatalytic processes. This assay was also applied to detect acetylcholine esterase inhibitors. Alkaline phosphatase hydrolyzes thiophosphate and yields H(2)S, which instantly reacts with Cd(2+) to give CdS quantum dots. The formation of CdS quantum dots in both reactions was followed by fluorescence spectroscopy and showed dependence on the concentration of enzyme and substrate.

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Sigma-Aldrich
硫酸镉, ACS reagent, ≥99.0%
Sigma-Aldrich
硫酸镉 8/3水合物, puriss. p.a., ACS reagent, ≥99.0% (calc. based on CdSO4 · 8/3 H2O, KT)