Continuous wavelet and derivative transform applied to the overlapping spectra for the quantitative spectrophotometric multi-resolution of triamterene and hydrochlorothiazide in triamterene-H tablets.

In present paper, continuous wavelet transform as well as first and second derivative spectrophotometry methods are proposed for simultaneous quantitative analysis of triamterene and hydrochlorothiazide without the time-consuming extraction step. In the wavelet transform method, different wavelet families were tested and subsequently coiflets wavelet family (coif1) and reverse biorthogonal wavelet family (rbio 2.8) were selected and applied under the optimal conditions for simultaneous analysis. The two above mentioned methods are based upon use of the continuous wavelet and derivative transforms of the spectra and measurement at zero-crossing wavelengths. Validation of the developed methods was confirmed by analyzing various synthetic mixtures of the investigated drugs. The experimental results obtained from the continuous wavelet transform approach were statistically compared with those yielded by derivative spectrophotometry methods and therefore led to successful results.