[Determination of aurantio-obtusin and chrysophanol in cassiae semen by HPLC].

To improve the determination method of Cassiae Semen in China Pharmacopeia edition 2005. MMETHOD: Aurantio-obtusin and chrysophanol were separated on an Inertsil ODS-3 column with acetonitrile-0.1% phosphoric acid solutiona as mobile phase in gradient elution. The linear ranges of aurantio-obtusin and chrysophanol were 2.3-230, 2.87-286 mg x L(-1), respectively. The average recover were 100.3% (RSD 3.1%), and 99.5% (RSD 2.5%) respectively. This method is specific, accurate and reproducible, and can be used for the assay of Cassiae Semen in the new edition of Chinese pharmacopoeia.