- Autofluorescence generation and elimination: a lesson from glutaraldehyde.
Autofluorescence generation and elimination: a lesson from glutaraldehyde.
Chemical communications (Cambridge, England) (2013-03-07)
Kwahun Lee, Sungmoon Choi, Chun Yang, Hai-Chen Wu, Junhua Yu
PMID23462727
摘要
Glutaraldehyde causes especially high autofluorescence. It reacted with proteins and peptides to generate visible to near-IR emitters. A model indicated that ethylenediamine and a secondary amine in the molecule were key components for the formation of emissive species. The mechanism enables us to control the generation and elimination of autofluorescence.
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Sigma-Aldrich
戊二醛 溶液, Grade I, 25% in H2O, specially purified for use as an electron microscopy fixative
Sigma-Aldrich
戊二醛 溶液, Grade I, 50% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
Sigma-Aldrich
戊二醛 溶液, Grade I, 70% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
Sigma-Aldrich
戊二醛 溶液, Grade I, 8% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use