[Studies on "Fast Green FCF Standard" for the dye standard on the National Institute of Health Sciences].

The raw material for Fast Green FCF was tested for preparation of the "Fast Green FCF Standard (C.I. 42053)". Analytical data obtained were as follows: paper chromatography, only one spot is observed; arsenic content, 0.38 microgram/g; chloride content, 0.11%; sulfate content, 3.30%; heavy metals, lead, 8.0 micrograms/g, manganese, 28.1 micrograms/g, and chromium, 1.6 micrograms/g; infrared spectra, 1575 cm-1, 1169 cm-1, and 1033 cm-1; loss on drying, 2.39%; assay, 93.0% by the titanium trichloride titration. Based on the above results, the raw material was authorized as the Dye Standard of National Institute of Health Sciences.