- Isolation and identification of a new bifidogenic growth stimulator produced by Propionibacterium freudenreichii ET-3.
Isolation and identification of a new bifidogenic growth stimulator produced by Propionibacterium freudenreichii ET-3.
Bioscience, biotechnology, and biochemistry (2002-05-15)
Kakuhei Isawa, Kenichi Hojo, Nobuo Yoda, Tomonori Kamiyama, Seiya Makino, Mizue Saito, Hitomi Sugano, Chinami Mizoguchi, Saori Kurama, Mika Shibasaki, Noriko Endo, Yoshio Sato
PMID12005071
摘要
We have found a new growth stimulator for bifidobacteria in the culture broth of Propionibacterium freudenreichii ET-3. The bifidogenic growth stimulator (BGS) was purified by Diaion HP-20 column chromatography and preparative HPLC. Spectroscopic methods including 1H-NMR, UV, and LC-ESI-MS experiments indicated that the chemical structure of the bifidogenic growth stimulator was 1,4-dihydroxy-2-naphthoic acid (DHNA). Approximately 10 mg/L of DHNA was found to be produced in the culture broth of P. freudenreichii ET-3.
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