Inhibition of the subgenomic hepatitis C virus replicon in huh-7 cells by 2'-deoxy-2'-fluorocytidine.

Inhibition of the subgenomic hepatitis C virus replicon in huh-7 cells by 2'-deoxy-2'-fluorocytidine.

Antimicrobial agents and chemotherapy (2004-01-27)

Lieven J Stuyver, Tamara R McBrayer, Tony Whitaker, Phillip M Tharnish, Mangala Ramesh, Stefania Lostia, Leanne Cartee, Junxing Shi, Ann Hobbs, Raymond F Schinazi, Kyoichi A Watanabe, Michael J Otto

PMID14742230

摘要

2'-Deoxy-2'-fluorocytidine (FdC) is a potent inhibitor of the hepatitis C virus RNA replicon in culture, and FdC-5'-triphosphate is an effective inhibitor of the NS5B polymerase. Dynamic profiling of cell growth in an antiviral assay showed that FdC caused cytostasis due to an S-phase arrest. These observations demonstrate that FdC treatment is affecting both a viral target and a cellular target.