[Isolation of 5-benzyl hydroxytryptophan enantiomers by using microbial aminoacylase].

[Isolation of 5-benzyl hydroxytryptophan enantiomers by using microbial aminoacylase].

Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic] (1988-01-01)

L T Kushashvili, A K Zhuravlev, A G Borodkin, G V Popova, A D Nekliudov

摘要

The process of asymmetric deacylation of an acetyl derivative of 5-benzyl hydroxytryptophan racemate in the presence of native microbial aminoacylase was studied. The effect of pH, temperature, concentration of the enzyme and substrate were investigated. Conditions for preparation and isolation of individual amino acids were defined by optimization of N-Ac-DU-5-BOT hydrolysis.