A novel modified carbon paste electrode based on NiO/CNTs nanocomposite and (9, 10-dihydro-9, 10-ethanoanthracene-11, 12-dicarboximido)-4-ethylbenzene-1, 2-diol as a mediator for simultaneous determination of cysteamine, nicotinamide adenine dinucleotide and folic acid.

A novel modified carbon paste electrode based on NiO/CNTs nanocomposite and (9, 10-dihydro-9, 10-ethanoanthracene-11, 12-dicarboximido)-4-ethylbenzene-1, 2-diol as a mediator for simultaneous determination of cysteamine, nicotinamide adenine dinucleotide and folic acid.

Biosensors & bioelectronics (2013-05-28)

Hassan Karimi-Maleh, Pourya Biparva, Mehdi Hatami

摘要

A carbon paste electrode (CPE) modified with (9, 10-dihydro-9, 10-ethanoanthracene-11, 12-dicarboximido)-4-ethylbenzene-1, 2-diol (DEDE) and NiO/CNTs nanocomposite was used for the sensitive voltammetric determination of cysteamine (CA), nicotinamide adenine dinucleotide (NADH) and folic acid (FA) for the first time. The synthesized materials were characterized with different methods such as XRD, cyclic voltammetry, electrochemical impedance spectroscopy (EIS) and square wave voltammetry (SWV). The modified electrode exhibited a potent and persistent electron mediating behavior followed by well-separated oxidation peaks of CA, NADH and FA. The peak currents were linearly dependent on CA, NADH and FA concentrations using square wave voltammetry (SWV) method in the ranges of 0.01-250, 1.0-500, and 3.0-550 µmol L⁻¹, with detection limits of 0.007, 0.6, and 0.9 µmol L⁻¹, respectively. The modified electrode was used for the determination of CA, NADH and FA in biological and pharmaceutical samples.

材料

货号

品牌

产品描述

296848

Sigma-Aldrich

乙苯, anhydrous, 99.8%

M9768

Sigma-Aldrich

半胱胺, ~95%

30070

Sigma-Aldrich

半胱胺, ≥98.0% (RT)

M6500

Sigma-Aldrich

半胱胺盐酸盐, ≥98% (titration)

203882

Sigma-Aldrich

氧化镍(II), 99.99% trace metals basis

03079

Supelco

乙苯, analytical standard

637130

Sigma-Aldrich

氧化镍(II), nanopowder, <50 nm particle size (TEM), 99.8% trace metals basis

E12508

Sigma-Aldrich

乙苯, ReagentPlus^®, 99%

30078

Sigma-Aldrich

半胱胺盐酸盐, BioXtra

399523

Sigma-Aldrich

氧化镍(II), green, −325 mesh, 99%

481793

Sigma-Aldrich

氧化镍(II), ≥99.995% trace metals basis

487104

Supelco

乙苯溶液溶液, NMR reference standard, 0.1% in chloroform-d (99.8 atom % D), TMS 0.01 %, NMR tube size 5 mm × 8 in.

30080

Sigma-Aldrich

半胱胺盐酸盐, ≥97.0% (RT)

708046

Sigma-Aldrich

乙苯溶液溶液, NMR reference standard, 10% in chloroform-d (99.8 atom % D)

40036

Supelco

乙苯溶液溶液, certified reference material, 5000 μg/mL in methanol

717959

Sigma-Aldrich

乙苯溶液溶液, NMR reference standard, 1% in chloroform-d (99.8 atom % D)

714917

Sigma-Aldrich

乙苯溶液溶液, NMR reference standard, 0.1% in chloroform-d (99.8 atom % D), TMS 0.01 %, NMR tube size 5 mm × 8 in. , ultra-thin wall

736198

Supelco

乙苯溶液溶液, NMR reference standard, 10% in chloroform-d (99.8 atom % D), NMR tube size 5 mm × 8 in. , ultra-thin wall

612065

Sigma-Aldrich

乙苯溶液溶液, NMR reference standard, 0.1% in chloroform-d (99.8 atom % D), TMS 0.01 %, NMR tube size 10 mm × 8 in.

708054

Sigma-Aldrich

乙苯溶液溶液, NMR reference standard, 0.1% in chloroform-d ("100%", 99.96 atom % D)

717967

Sigma-Aldrich

乙苯溶液溶液, NMR reference standard, 0.1% in chloroform-d (99.8 atom % D), TMS 0.1 %

551341

Sigma-Aldrich

乙苯溶液溶液, NMR reference standard, 5% in chloroform-d (99.8 atom % D), TMS 2 %, NMR tube size 5 mm × 8 in.

710725

Sigma-Aldrich

乙苯溶液溶液, NMR reference standard, 5% in chloroform-d (99.8 atom % D), TMS 1 %, NMR tube size 5 mm × 7 in.

487120

Sigma-Aldrich

乙苯溶液溶液, NMR reference standard, 0.1% in chloroform-d (99.8 atom % D), TMS 0.01 %, NMR tube size 8 mm × 8 in.

733768

Sigma-Aldrich

乙苯溶液溶液, NMR reference standard, 10% in chloroform-d (99.8 atom % D), NMR tube size 10 mm × 8 in.

612480

Supelco

乙苯溶液溶液, NMR reference standard, 0.1% in chloroform-d (99.8 atom % D), chromium(III) acetylacetonate 0.1 mg/mL, TMS 0.01 %, NMR tube size 5 mm × 8 in.

V900342

Sigma-Aldrich

半胱胺盐酸盐, Vetec^™, reagent grade, 98%