A colloidal gold nanoparticle-based immunochromatographic test strip for rapid and convenient detection of Staphylococcus aureus.

A colloidal gold nanoparticle-based immunochromatographic test strip for rapid and convenient detection of Staphylococcus aureus.

Journal of nanoscience and nanotechnology (2014-04-25)

Kaili Niu, Xiaoping Zheng, Chusen Huang, Kuan Xul, Yuan Zhi, Hebai Shen, Nengqin Jia

摘要

An immunochromatographic test strip using gold nanoparticles-staphylococcus aureus monoclonal antibody conjugates was developed for the rapid and convenient detection of staphylococcus aureus based on a double-antibody sandwich format. The detection limit and the detection rate of this test strip is 10(3) CFU /mL and 98.7%, respectively. It could be used for the rapid detection of staphylococcus aureus in food and the results can be visually identified by the naked eye within 10 min. Compared with conventional bacterial detection methods, this developed immunochromatographic assay based test strip has several advantages including simple, fast, low-cost, favorable sensitivity and specificity, exhibiting a great potential for application in food safety control systems and clinical diagnosis.

材料

Product Number

品牌

产品描述

265772

Sigma-Aldrich

金, powder, <45 μm, 99.99% trace metals basis

643246

Sigma-Aldrich

镀金显微镜载片, layer thickness 1000 Å, 99.999% (Au)

GF96742479

金, rod, 50mm, diameter 3.0mm, as drawn, 99.95%

265780

Sigma-Aldrich

金, wire, diam. 0.25 mm, 99.99% trace metals basis

GF77657574

金, sphere, 20pcs, diameter 0.5mm, 99.99%

GF32175569

金, insulated wire, 1m, conductor diameter 0.025mm, insulation thickness 0.005mm, polyester insulation, 99.99%

GF41388767

金, insulated wire, 2m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyimide insulation, 99.99%

GF06766713

金, insulated wire, 5m, conductor diameter 0.125mm, insulation thickness 0.014mm, polyester insulation, 99.99%

GF49538469

金, insulated wire, 2m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyester insulation, 99.99%

GF33293729

金, insulated wire, 0.1m, conductor diameter 0.125mm, insulation thickness 0.016mm, PTFE (polytetrafluoroethylene) insulation, 99.99%

GF08843867

金, rod, 10mm, diameter 3.0mm, as drawn, 99.95%

GF50454132

金, insulated wire, 2m, conductor diameter 0.125mm, insulation thickness 0.014mm, polyester insulation, 99.99%

GF31080899

金, rod, 25mm, diameter 2.0mm, as drawn, 99.95%

GF11444611

金, insulated wire, 5m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyimide insulation, 99.99%

GF88214542

金, insulated wire, 1m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyimide insulation, 99.99%

GF12231223

金, microleaf disks, 10mm, thinness 0.25μm, specific density 483μg/cm², removable support, 99.99+%

GF39029968

金, rod, 100mm, diameter 2.0mm, as drawn, 99.95%

GF63042870

金, rod, 10mm, diameter 5.0mm, as drawn, 99.95%

GF43173930

金, rod, 25mm, diameter 3.0mm, as drawn, 99.95%

GF86990350

金, tube, 100mm, outside diameter 2.0mm, inside diameter 1.8mm, wall thickness 0.1mm, as drawn, 99.95%

GF22371319

金, rod, 25mm, diameter 4.0mm, as drawn, 99.95%

GF85112232

金, rod, 50mm, diameter 2.0mm, as drawn, 99.95%

GF72963221

金, insulated wire, 0.5m, conductor diameter 0.125mm, insulation thickness 0.014mm, polyester insulation, 99.99%

GF23782350

金, microfoil, disks, 10mm, thinness 0.1μm, specific density 204.1μg/cm², permanent mylar 3.5μm support, 99.99+%

GF38598980

金, microfoil, disks, 10mm, thinness 0.5μm, specific density 966μg/cm², permanent mylar 3.5μm support, 99.99+%

GF03557336

金, rod, 10mm, diameter 6.0mm, as drawn, 99.95%

GF47078129

金, microleaf disks, 10mm, thinness 0.5μm, specific density 966μg/cm², removable support, 99.99+%

GF98812097

金, rod, 6mm, diameter 6.0mm, as drawn, 99.95%

GF59949264

金, tube, 200mm, outside diameter 1.0mm, inside diameter 0.5mm, wall thickness 0.25mm, as drawn, 99.95%

GF62399133

金, insulated wire, 0.5m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyimide insulation, 99.99%