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Merck
CN
  • Natural and artificial cystine knots for assembly of homo- and heterotrimeric collagen models.

Natural and artificial cystine knots for assembly of homo- and heterotrimeric collagen models.

Antioxidants & redox signaling (2007-10-27)
Cyril Boulègue, Hans-Jürgen Musiol, Marion G Götz, Christian Renner, Luis Moroder
摘要

Native collagens are molecules that are difficult to handle because of their high tendency towards aggregation and denaturation. It was discovered early on that synthetic collagenous peptides are more amenable to conformational characterization and thus can serve as useful models for structural and functional studies. Single-stranded collagenous peptides of high propensity to self-associate into triple-helical trimers were used for this purpose as well as interchain-crosslinked homotrimers assembled on synthetic scaffolds. With the growing knowledge of the biosynthetic pathways of natural collagens and the importance of their interchain disulfide crosslinks, which stabilize the triple-helical structure, native as well as de novo designed cystine knots have gained increasing attention in the assembly of triple-stranded collagen peptides. In addition, natural sequences of collagens were incorporated in order to biophysically characterize their functional epitopes. This review is focused on the methods developed over the years, and future perspectives for the production of collagen-mimicking synthetic and recombinant triple-helical homo- and heterotrimers.

材料
Product Number
品牌
产品描述

Sigma-Aldrich
L -胱氨酸, from non-animal source, meets EP testing specifications, suitable for cell culture, 98.5-101.0%
SAFC
L -胱氨酸
Sigma-Aldrich
L -胱氨酸, ≥98% (TLC), crystalline
Sigma-Aldrich
L -胱氨酸, ≥99.7% (TLC)
Supelco
L-胱氨酸, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
L -胱氨酸, Wacker Chemie AG, ≥98.5%
胱氨酸, European Pharmacopoeia (EP) Reference Standard
Supelco
L -胱氨酸, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland