The cloning of PIG-A, a component in the early step of GPI-anchor biosynthesis.

The cloning of PIG-A, a component in the early step of GPI-anchor biosynthesis.

Science (New York, N.Y.) (1993-02-26)

T Miyata, J Takeda, Y Iida, N Yamada, N Inoue, M Takahashi, K Maeda, T Kitani, T Kinoshita

摘要

The glycosylphosphatidylinositol (GPI) anchor is a membrane attachment structure of many proteins and occurs in a wide variety of eukaryotes from yeasts to mammals. The structure of the core of the GPI anchor is conserved in protozoa and mammals and so is its biosynthetic pathway. A complementary DNA encoding a human protein termed PIG-A (phosphatidylinositol glycan-class A) was cloned. PIG-A was necessary for synthesis of N-acetylglucosaminyl-phosphatidylinositol, the very early intermediate in GPI-anchor biosynthesis.