Efficient synthesis of O-antigen fragments expressed by Burkholderia anthina by modular synthesis approach.

To facilitate mapping of the interaction region of the O-chain of the lipopolysaccharide from Burkholderia anthina and of a lipopolysaccharide-specific monoclonal antibody, trisaccharide propyl α-L-rhamnopyranosyl-(1 → 2)-α-D-galactopyranosyl-(1 → 3)-α-L-rhamnopyranoside (27) and hexasaccharide propyl α-L-rhamnopyranosyl-(1 → 2)-α-D-galactopyranosyl-(1 → 3)-α-L-rhamnopyranosyl-(1 → 2)-α-L-rhamnopyranosyl-(1 → 2)-α-D-galactopyranosyl-(1 → 3)-α-L-rhamnopyranoside (33) were synthesized. These oligosaccharides represent the repeating monomer and dimer of the O-antigen, respectively.