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Merck
CN
  • Defined nanoscale chemistry influences delivery of peptido-toxins for cancer therapy.

Defined nanoscale chemistry influences delivery of peptido-toxins for cancer therapy.

PloS one (2015-06-02)
Santosh K Misra, Mao Ye, Sumin Kim, Dipanjan Pan
摘要

We present an in-silico-to-in-vitro approach to develop well-defined, self-assembled, rigid-cored polymeric (Polybee) nano-architecture for controlled delivery of a key component of bee venom, melittin. A competitive formulation with lipid-encapsulated (Lipobee) rigid cored micelle is also synthesized. In a series of sequential experiments, we show how nanoscale chemistry influences the delivery of venom toxins for cancer regression and help evade systemic disintegrity and cellular noxiousness. A relatively weaker association of melittin in the case of lipid-based nanoparticles is compared to the polymeric particles revealed by energy minimization and docking studies, which are supported by biophysical studies. For the first time, the authors' experiment results indicate that melittin can play a significant role in DNA association-dissociation processes, which may be a plausible route for their anticancer activity.

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Sigma-Aldrich
四氢呋喃, anhydrous, ≥99.9%, inhibitor-free
Sigma-Aldrich
四氢呋喃, anhydrous, contains 250 ppm BHT as inhibitor, ≥99.9%
Sigma-Aldrich
蜂毒肽 来源蜜蜂毒液, ≥85% (HPLC)
Sigma-Aldrich
蜂毒肽 来源蜜蜂毒液, ≥65% (HPLC)
Sigma-Aldrich
四氢呋喃, inhibitor-free, purification grade