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Paracoccus aquimaris sp. nov., isolated from seawater.

Antonie van Leeuwenhoek (2015-08-08)
Kunho Kim, Sang-Seob Lee
摘要

A Gram-stain negative, motile and short rod-shaped bacterial strain, designated KF89(T), was isolated from seawater of Tong-Yeong, South of Korea. Growth occurred between 10 and 35 °C (optimum 32 °C) and at pH 6.0-9.0 (optimum pH 7.0) and at 0-7 % NaCl (optimum at 2 % NaCl). The 16S rRNA gene sequence identified it as a member of the genus Paracoccus that belongs to the phylum Proteobacteria. Based on the 16S rRNA gene sequence, the highest degree of gene sequence similarities were shared with Paracoccus homiensis DD-R11(T) (97.3 %), P. zeaxanthinifaciens ATCC21588(T) (97.1 %), P. rhizosphaerae CC-CCM15-8(T) (96.9 %), P. beibuensis JLT1284 (96.9 %) and P. aestuarii B7(T) (96.6 %). Strain KF89(T) contained Ubiquinone-10 as the major respiratory quinone, and the polar lipid profile included phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol and an unidentified aminolipid. The major fatty acid of strain KF89(T) is C18:1 ω7c and the DNA G+C content is 57 mol%. Strain KF89(T) showed a DNA-DNA relatedness with P. homiensis KACC 11518(T) of 49 %. Based on phylogenetic and phenotypic analyses, strain KF89(T) is clearly shown to be a novel member of the genus Paracoccus. The type strain is Paracoccus aquimaris KF89(T) (=KEMB 3-508(T) = JCM 19892(T)).

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Sigma-Aldrich
磷钼酸 水合物 溶液, 20 wt. % in ethanol
Supelco
磷钼酸 水合物, ≥99.99% trace metals basis
Sigma-Aldrich
磷钼酸 水合物 溶液, spray reagent, 10% in ethanol