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SiR-Hoechst is a far-red DNA stain for live-cell nanoscopy.

SiR-Hoechst is a far-red DNA stain for live-cell nanoscopy.

Nature communications (2015-10-02)

Gražvydas Lukinavičius, Claudia Blaukopf, Elias Pershagen, Alberto Schena, Luc Reymond, Emmanuel Derivery, Marcos Gonzalez-Gaitan, Elisa D'Este, Stefan W Hell, Daniel Wolfram Gerlich, Kai Johnsson

摘要

Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utility. Here we describe a far-red DNA stain, SiR-Hoechst, which displays minimal toxicity, is applicable in different cell types and tissues, and is compatible with super-resolution microscopy. The combination of these properties makes this probe a powerful tool for live-cell imaging.

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