- Rapid preconcentration of viable bacteria using magnetic ionic liquids for PCR amplification and culture-based diagnostics.
Rapid preconcentration of viable bacteria using magnetic ionic liquids for PCR amplification and culture-based diagnostics.
Analytical and bioanalytical chemistry (2017-06-22)
Kevin D Clark, Jeffrey A Purslow, Stephen A Pierson, Omprakash Nacham, Jared L Anderson
PMID28634762
摘要
In this study, a series of magnetic ionic liquids (MILs) were investigated for the extraction and preconcentration of bacteria from aqueous samples. By dispersing small volumes (e.g., 15 μL) of MIL within an aqueous cell suspension, bacteria were rapidly extracted and isolated using a magnetic field. Of the seven hydrophobic MILs examined, the trihexyl(tetradecyl)phosphonium Ni(II) hexafluoroacetylacetonate ([P