- Phenotypic and functional characterization of in vitro cultured human mast cells.
Phenotypic and functional characterization of in vitro cultured human mast cells.
Cytometry. Part B, Clinical cytometry (2016-07-13)
N Cop, I I Decuyper, M A Faber, V Sabato, C H Bridts, M M Hagendorens, B Y De Winter, L S De Clerck, D G Ebo
PMID27401129
摘要
Mast cell progenitor cells, derived from CD34 Human mast cells (HuMC) were cultured from peripheral blood, phenotypically characterized, passively sensitized with allogenic IgE antibodies and finally stimulated by anti-IgE that crosslinks IgE/FcεRI complexes. Alterations in the number of cells positive for CD63 and release of histamine were quantified simultaneously by flow cytometry. In culture, two distinct CD45 This study discloses that culturing HuMC from CD34
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