- Improved therapeutic efficacy of mammalian expressed-recombinant interferon gamma against ovarian cancer cells.
Improved therapeutic efficacy of mammalian expressed-recombinant interferon gamma against ovarian cancer cells.
Experimental cell research (2017-08-15)
Ali Razaghi, Carina Villacrés, Vincent Jung, Narges Mashkour, Michael Butler, Leigh Owens, Kirsten Heimann
PMID28803068
摘要
Human interferon gamma (hIFNγ) affects tumour cells and modulates immune responses, showing promise as an anti-cancer biotherapeutic. This study investigated the effect of glycosylation and expression system of recombinant hIFNγ in ovarian carcinoma cell lines, PEO1 and SKOV3. The efficacy of E. coli- and mammalian-expressed hIFNγ (hIFNγ-CHO and HEK293, glycosylated/de-glycosylated) on cytostasis, cell death (MTT, and Guava-ViaCount