Kinetic modeling predicts a stimulatory role for ribosome collisions at elongation stall sites in bacteria.

Kinetic modeling predicts a stimulatory role for ribosome collisions at elongation stall sites in bacteria.

eLife (2017-05-13)

Michael A Ferrin, Arvind R Subramaniam

摘要

Ribosome stalling on mRNAs can decrease protein expression. To decipher ribosome kinetics at stall sites, we induced ribosome stalling at specific codons by starving the bacterium

材料

产品编号

品牌

产品描述

F3165

Sigma-Aldrich

单克隆抗-FLAG^® M2 小鼠抗, clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)