Simultaneous extraction and reverse-phase high-performance liquid chromatographic determination of adenine and pyridine nucleotides in human red blood cells.

Simultaneous extraction and reverse-phase high-performance liquid chromatographic determination of adenine and pyridine nucleotides in human red blood cells.

Analytical biochemistry (1985-04-01)

V Stocchi, L Cucchiarini, M Magnani, L Chiarantini, P Palma, G Crescentini

摘要

A simple and rapid method for the determination of ATP, ADP, AMP, NADP+, NAD+, NADPH, and NADH in human erythrocytes is described. A single-step extraction procedure employing alkaline medium and CF 50A Amicon ultrafiltration membranes allows a simultaneous and total recovery of the compounds of interest. Analysis is performed by reverse-phase high-performance liquid chromatography on a 5-micron Supelcosil LC-18 column and uv detection. Extraction and analysis require about 30 min. Levels of adenine and pyridine nucleotides in normal adults are also presented.

材料

Product Number

品牌

产品描述

58928-U

Supelco

SUPELCOSIL ^™ LC-18-S HPLC 色谱柱, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

59564

Supelco

SUPELCOSIL^™ LC-18 Supelguard 柱芯, 5 μm particle size, L × I.D. 2 cm × 4 mm

59132

Supelco