Purostar RP18E 2MYM HI-HR100-2.1 mm (1.50648.0001)
[A] 95/5 water / acetonitrile in 0.1% phosphoric acid; [B] 5/95 water/acetonitrile in 0.1% phosphoric acid
0% B for 2 min, then 0% to 100% B in 10 min; 100% to 0% B in 0.1 min; hold at 0% B for 4.9 min.
0.3 mL/min
4656 psi (321 bar)
30 °C
210 nm UV
1 μL
100 μg/mL of lidocaine, related compound A and H in acetonitrile
Lidocaine is a medication used to numb tissue in a specific area and does not affect other areas. Topically it can be used for reducing pain by irritations such as sunburn, poison ivy, insect bites, minor cuts or even hemorrhoids. It can also be used for local anesthetic with sedative, analgesic and cardiac depressant (antiarrhythmic drug) properties This application demonstrates reverse phase interactions in a simple gradient separating lidocaine and two of its related compounds using Cerilliant CRM′s for the related compounds.
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany