biological source
mouse breast
packaging
tube of 5 μg 90111911-DNA-5UG, pkg of vial of cells 90111911-1VL
growth mode
Adherent
karyotype
2n = 40; diploid
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity and virus studies: Bittner virus
Biochem/physiol Actions
Derived from a spontaneous mammary tumour which arose in a C57BL x A/F1 hybrid female mouse. Bittner virus has been revealed in the cells by electron microscopy. Tumourigenic in BALB/c mice.
Mouse C57BL x A/F1 mammary tumour
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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法规信息
低风险生物材料
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