生物来源
mouse breast
包装
tube of 5 μg 90111911-DNA-5UG
pkg of vial of cells 90111911-1VL
生长模式
Adherent
核型
2n = 40; diploid
形态学
Epithelial-like
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
细胞系来源
Mouse C57BL x A/F1 mammary tumour
细胞系描述
Derived from a spontaneous mammary tumour which arose in a C57BL x A/F1 hybrid female mouse. Bittner virus has been revealed in the cells by electron microscopy. Tumourigenic in BALB/c mice.
应用
Tumourigenicity and virus studies: Bittner virus
培养基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
其他说明
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