The GenElute™ PCR Clean-up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reaction, such as excess primers, nucleotides, DNA polymerase, oil and salts. DNA purification is achieved in a few easy steps. Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.
Sufficient for 70 purifications.
Insert the binding column into a provided collection tube.
Add 0.5 mL of the column preparation solution to each miniprep column.
Centrifuge at 12,000 x g for 1 minute.
After 1 minute, discard the eluate.
Transfer PCR reaction mix into each tube.
Add 5 volumes of binding solution to 1 volume of the PCR reaction and mix.
Transfer the solution into the binding column.
Centrifuge at maximum speed for 1 minute.
After 1 minute, discard the eluate, but retain the collection tube.
Add 0.5 mL of diluted wash solution to the column.
Centrifuge at maximum speed for 1 minute.
After 1 minute, discard the eluate, replace the column back into the collection tube.
Centrifuge at maximum speed for 2 minutes (without adding additional wash solution.
After 2 minutes, discard any residual eluate and the collection tube. Transfer the column to a fresh 2 mL collection tube.
Add 50 µL of elution solution to the center of each column.
Centrifuge at maximum speed for 1 minute.
After 1 minute, discard the column.
PCR amplification products are now presented in the eluate.
如要继续阅读,请登录或创建帐户。
暂无帐户?