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UNSPSC Code:
41106514
Biological source:
human blood
Technique(s):
cell culture | mammalian: suitable
Ethnicity:
Caucasian
Gender:
male
Growth mode:
Suspension
Morphology:
Lymphoblastoid
biological source
human blood
packaging
tube of 5 μg 94082244-DNA-5UG, pkg of vial of cells 94082244-1VL
ethnicity
Caucasian
gender
male
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
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General description
Consanguineous: No
Homozygous: No
Biochem/physiol Actions
A: 01 68
B: 08 40
HLA DRB1: 0301 0401
HLA DRB3: 101
HLA DRB4: 101
HLA DQAP: 3.1 4.1
HLA DQA1: 0501 03
HLA DQB1: 0201 0301
HLA DPB1: 0101 0301
B: 08 40
HLA DRB1: 0301 0401
HLA DRB3: 101
HLA DRB4: 101
HLA DQAP: 3.1 4.1
HLA DQA1: 0501 03
HLA DQB1: 0201 0301
HLA DPB1: 0101 0301
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
Preparation Note
RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.
Other Notes
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HLA A: 1 28
HLA A ief:
HLA C: 3
HLA B: 8 60
HLA A ief:
HLA C: 3
HLA B: 8 60
Disclaimer
This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.
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