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Universal ProbeLibrary System Technology
UPL is based on only 165 short hydrolysis probes. They are labeled at the 5' end with fluorescein (FAM) and at the 3' end with a dark quencher dye.
Troubleshooting PCR and RT-PCR Amplification
This page shows PCR and RT-PCR amplification troubleshooting.
Kapa Biosystems qPCR Reagents and Kits
Quantitative PCR (qPCR) and qRT-PCR reagents and kits that are designed to deliver maximum efficiency and reliable results from a variety of genetic materials.
Primer and Probe Design
Video: Primer and Probe Design
PCR Assay Optimization and Validation
PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.
Complete Solutions for PCR Assay Development
Fit-for-use products offer the quality, consistency & documentation necessary for every step of your IVD development and manufacturing process.
Custom Oligos Modifications
We offer over 200 custom DNA oligos modification for both DNA, RNA oligonucleotides and sequencing to probes for gene.
PCR Technologies Guide
Basic PCR/qPCR/dPCR protocols for assay quality control and specific studies.
Array CGH Analysis of Challenging Samples
aCGH technology enhances chromosomal analysis, requiring significant DNA input per sample.
Probe-based qPCR
Probe-based QPCR offers high sensitivity and specificity with fluorescent-labeled probes and various dye options for multiplex amplification.
Molecular Beacons
Learn about precision Sigma-Aldrich Molecular Beacon oligonucleotide probes used for sequence detection in qPCR for in vitro research.
Probe QPCR for Gene-Level Expression
qPCR investigates gene expression, amplification, and alterations, crucial for tumor biology and understanding cancer genetics.
Choosing the Right Custom qPCR Probe
View a summary of the applications using commonly ordered custom qPCR probes.
DNA Oligonucleotide Synthesis
Learn about the steps in phosphoramidite solid-phase method of Oligonucleotide Synthesis.
PCR/qPCR Data Analysis
After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.
PCR/qPCR Data Analysis
After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.
Oligonucleotide Quality Control by Mass Spectrometry
Mass Spectrometry is the technology of choice for analyzing oligonucleotide synthesis. It enables the most sensitive detection of low levels of by-products, which can affect performance.
Mgat4 May Play a Role in Increased Sialylation by Overexpressing Functional MGAT1 in Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells
MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.
Quantitative RT-PCR
RT-qPCR products combine the effective of Reverse Transcriptase with hot-start taq-directed antibody in convenient ReadyMixes for probe-based or SYBR® Green based applications.
Direct and Crude Extract qPCR
Rapid qPCR analysis from highly inhibited tissue and blood sample extractions with KAPA PROBE.
RNA Immunoprecipitation (RIP) FAQs
RNA Immunoprecipitation (RIP) Frequently Asked Questions
KAPA SYBR® FAST qPCR Kits FAQs
Review some of the most asked questions regarding the KAPA SYBR FAST qPCR kits, which provide optimal performance under strict real-time PCR reaction conditions, and improve the signal-to-noise ratio (fluorescence), quantitation cycle (Cq), linearity and sensitivity.
RT-PCR Troubleshooting
PCR troubleshooting guide for reliable data in molecular assays.
How qPCR Works
Watch these videos to learn how real time or quantitative PCR (qPCR) works and the benefits of both the SYBR Green-based and probe-based methods of qPCR assay.
PCR/qPCR/dPCR Assay Design
PCR workflow's variability influenced by sample source, reverse transcription, and assay design, impacting reproducibility.
MIQE: Real-Time PCR Experiment Standards
MIQE-compliant qPCR services support primer design, protocol development, and data analysis for faster product development and publication.
KiCqStart® SYBR® Green Primers FAQs
Primer pairs detect prevalent eukaryotic genes, but may co-amplify other transcripts, emphasizing the importance of comprehensive primer design.
Oligonucleotide Quantification
DNA can be easily quantitated in a UV spectrometer due to its highly conjugated nature.
Oligonucleotide Quantification
DNA can be easily quantitated in a UV spectrometer due to its highly conjugated nature.
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