repeat pipettes

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Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)

Human Aortic Endothelial Cells (HAOEC) Culture Protocol

Technical information for working with human aortic endothelial cells including thawing, subculturing and cryopreservation.

How to Use BRAND® Pipettes

This page contains detailed information on working with and handling pipettes and pipetting aids.

Plant RNA/DNA Purification Kit

The Plant RNA/DNA Purification Kit provides a rapid method for the isolation and purification of total RNA and DNA simultaneously from a single sample of plants.

Plant/Fungi Total RNA Purification Kit

The Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA from a wide range of plant and filamentous fungal species.

Enzymatic Assay of Lyticase

This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate.

Magnetic Bead-Based Purification/Screening Using His Mag Sepharose® Ni

This page shows magnetic bead-based purification and screening using His Mag Sepharose Ni from Cytiva.

Cell Culture Protocol 5: Subculture of Suspension Cell Lines

Cell culture protocol for passaging and splitting suspension cell lines

Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.

Preparing Cell Lysates

This page describes common challenges encountered when lysing cells and extracting proteins prior to Western blotting. Total protein concentration must be determined for these cell lysates. Variables affecting each of these steps are outlined below, as each could affect the

GenElute™ Plant Genomic DNA Miniprep Kit Protocol (G2N10, G2N70, G2N350)

This protocol describes a simple and convenient procedure to isolate pure DNA from a variety of plant species using the GenElute Plant Genomic DNA Miniprep Kit.

Thawing of Frozen Cell Lines

Cell culture protocol for proper thawing of cryopreserved cell lines.

HandyStep® Troubleshooting: Error 003

This video describes the HandyStep® Troubleshooing Error 003.

In Vitro Differentiation of Human iPS Cells Into Colon Organoids in Serum-Free Cell Culture Conditions

Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells.

HandyStep^® Troubleshooting: Error 001

How to clear errors from HandyStep® electronic Repeating Pipette. While error occurs on HandyStep® screen, push Enter key and screen will assist you in clearing the error.

Passaging Human iPS Cells Protocol Using EZ-LiFT™ Reagent

Human ips cell passaging protocol using EZ-LiFT™ Reagent, an enzyme-free and chemically defined pluripotent stem cell dissociation reagent.

Enzymatic Assay of Superoxide Dismutase

Transforming E.coli with Engineered Plasmid

Making Competent Cells; Making Agar Plates; Bacterial Transformation; Picking Colonies; Growing Bacteria in Liquid Culture; Freezing Bacteria.

Two solvents (Dioxane, DMAc), Strong Base (NaOt-Bu) Step-by-Step Guide for Buchwald-Hartwig Amination Reaction Screening Kit

Protocol for the KitAlysis™ Buchwald-Hartwig Amination Reaction Screening Kit

Plant DNA Extraction & WGA Amplification Protocol

GenomePlex® Whole Genome Amplification has been used to amplify genomic DNA from soybean, corn, tomato, purple coneflower, and ginseng.

Reliable DNA extraction from Whatman™ FTA cards

This study examined the yield and quality of DNA from samples applied to Whatman FTA cards, using five common methods of DNA extraction.

Plant/Fungi DNA Isolation Kit Protocol

The Plant/Fungi DNA Isolation Kit provides a rapid method for the isolation and purification of total DNA from a wide range of plant and fungi species.

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

Assay Procedure for Invertase

The appearance of reducing sugars is measured by the modified Fehling-Lehmann-Schoorl method.

Pull-down assays

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.

Enzymatic Assay of Lipase Type XIII from Pseudomonas sp. Using a Coupled Enzyme System of Glycerol Kinase and Glycerophosphate Oxidase (EC 3.1.1.3)

Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)

Standard PCR Protocol

Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.

Cultivating Consistency: Tips and Tricks for Successful Cell Cultures

Properly optimizing workflows and utilizing the latest best practices are the keys to obtaining consistent results and advancing research.

Cultrex^® 3-D Culture Matrix™ Laminin I Protocol

3-D Culture Matrix™ Mouse Laminin I may be used as a gel or as a media additive to study cellular growth and differentiation in three dimensions in vitro.

Enzymatic Assay of Esterase

Objective: To standardize a procedure for the enzymatic determination of Esterase activity using Ethyl Butyrate as a substrate.

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