- Capillary electrophoresis with field-amplified sample stacking for rapid and sensitive determination of sulfadiazine and sulfamethoxazole.
Capillary electrophoresis with field-amplified sample stacking for rapid and sensitive determination of sulfadiazine and sulfamethoxazole.
Die Pharmazie (2012-09-29)
Yuqin Li, Yingjie Cui, Baoxiu Jia, Hao Wang, Caihong Liu, Yongxiu Qi
PMID23016449
ABSTRACT
A new capillary electrophoresis method with field-amplified sample stacking (FASS) was developed for the analysis of sulfadiazine and sulfamethoxazole. After optimization of the separation and concentration conditions, the two compounds can be separated within 7 min and quantified with high sensitivity, with detection limits of 0.48 ng/mL for sulfadiazine and 0.76 ng/mL for sulfamethoxazole. This resulted in a 300-1500-fold improvement in concentration sensitivity relative to conventional capillary electrophoresis methods. The method was useful for qualitative and quantitative analysis of sulfadiazine and sulfamethoxazole in their preparations with recovery of 99.0%-102% for sulfadiazine and 99.5% - 99.7% for sulfamethoxazole.
MATERIALS
Product Number
Brand
Product Description
Supelco
Phosphate Standard for IC, TraceCERT®, 1000 mg/L phosphate in water (nominal concentration)
Supelco
Sulfamethoxazole, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sigma-Aldrich
Sodium phosphate dibasic, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99.0%, free-flowing, Redi-Dri™
Sigma-Aldrich
Sodium phosphate dibasic, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, E 339, anhydrous, 98-100.5% (calc. to the dried substance)
Sigma-Aldrich
Sodium phosphate dibasic dodecahydrate, meets analytical specification of Ph. Eur., BP, E339, 98.5-102.5% (T)