Cell patterning on polylactic acid through surface-tethered oligonucleotides.

Cell patterning on polylactic acid through surface-tethered oligonucleotides.

Acta biomaterialia (2014-12-03)

Toshiki Matsui, Yusuke Arima, Naohiro Takemoto, Hiroo Iwata

ABSTRACT

Polylactic acid (PLA) is a candidate material to prepare scaffolds for 3-D tissue regeneration. However, cells do not adhere or proliferate well on the surface of PLA because it is hydrophobic. We report a simple and rapid method for inducing cell adhesion to PLA through DNA hybridization. Single-stranded DNA (ssDNA) conjugated to poly(ethylene glycol) (PEG) and to a terminal phospholipid (ssDNA-PEG-lipid) was used for cell surface modification. Through DNA hybridization, modified cells were able to attach to PLA surfaces modified with complementary sequence (ssDNA'). Different cell types can be attached to PLA fibers and films in a spatially controlled manner by using ssDNAs with different sequences. In addition, they proliferate well in a culture medium supplemented with fetal bovine serum. The coexisting modes of cell adhesion through DNA hybridization and natural cytoskeletal adhesion machinery revealed no serious effects on cell growth. The combination of a 3-D scaffold made of PLA and cell immobilization on the PLA scaffold through DNA hybridization will be useful for the preparation of 3-D tissue and organs.

MATERIALS

Product Number

Brand

Product Description

725226

Sigma-Aldrich

6-Mercapto-1-hexanol, 99%

471283

Sigma-Aldrich

Triethylamine, ≥99.5%

02487

Supelco

Chloroform, analytical standard

451088

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6-Mercapto-1-hexanol, 97%

275611

Sigma-Aldrich

Rhein, technical grade

76887

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1,4-Dioxane, analytical standard

90340

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Triethylamine, puriss. p.a., ≥99.5% (GC)

90338

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Triethylamine, ampule, ≥99.5% (GC)

296309

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1,4-Dioxane, anhydrous, 99.8%, contains <=25 ppm BHT as stabilizer

372978

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Chloroform, anhydrous, contains amylenes as stabilizer, ≥99%

90337

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Triethylamine, for amino acid analysis, ≥99.5% (GC)

90335

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Triethylamine, BioUltra, ≥99.5% (GC)

F4274

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Fluorescein isothiocyanate isomer I, ≥97.5% (HPLC)

C7559

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Chloroform, ≥99%, PCR Reagent, contains amylenes as stabilizer

R7269

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Rhein

T0886

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Triethylamine, ≥99%

F7250

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Fluorescein isothiocyanate isomer I, suitable for protein labeling, ≥90% (HPLC), powder

288306

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Chloroform, anhydrous, ≥99%, contains 0.5-1.0% ethanol as stabilizer

319988

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366919

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Chloroform, ACS spectrophotometric grade, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer

24004

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Diethyl ether, puriss., contains ~5 mg/L 2,6-di-tert.-butyl-4-methylphenol as stabilizer, meets analytical specification of Ph. Eur., BP, ≥99.5% (GC)

33147

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1,4-Dioxane, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)

676934

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C2432

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