Skip to Content
Merck
CN
  • Dicer nuclease-promoted production of Let7a-1 microRNA is enhanced in the presence of tryptophan-containing amphiphilic peptides.

Dicer nuclease-promoted production of Let7a-1 microRNA is enhanced in the presence of tryptophan-containing amphiphilic peptides.

Chembiochem : a European journal of chemical biology (2014-07-06)
Soonsil Hyun, Areum Han, Myung Hyun Jo, Sungchul Hohng, Jaehoon Yu
ABSTRACT

A library of Trp-containing amphiphilic peptides was synthesized and screened for the ability to bind to pre-miRNA targets. Two members of this family, peptides Ac-WKKLLKWLKKLLKLAG-NH2 (2 b) and Ac-WKKLLKWLKKLLDabLAG-NH2 (4 b) were found to have nanomolar binding affinities to pre-let7a-1. Peptides 2 b and 4 b caused an increase in the in vitro Dicer cleavage of pre-let7a-1. This observation was confirmed by a cell-based assay, the results of which show an up to 50 % increase in Dicer activity. Enhanced expression of let7a-1 promoted by the peptides results in specific reductions of target mRNAs. The results of a microarray study show that lower amount of fluctuating genes are generated in the presence of 2 b or 4 b, relative to that from exogenous delivery of let7a-1. Because peptides 2 b and 4 b promote enhanced formation of mature let7a-1 only at the endogenous miRNA level, this specifically controls genes related to let7a-1.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
MOPS, BioUltra, Molecular Biology, ≥99.5% (titration)
Sigma-Aldrich
Formamide, BioUltra, Molecular Biology, ≥99.5% (T)
Sigma-Aldrich
Formamide, ACS reagent, ≥99.5%
Sigma-Aldrich
Formamide, ≥99.5% (GC), BioReagent, Molecular Biology
Sigma-Aldrich
MOPS, ≥99.5% (titration)
Sigma-Aldrich
MOPS, BioXtra, ≥99.5% (titration)
Sigma-Aldrich
MOPS, BioPerformance Certified, suitable for cell culture, ≥99.5% (titration)
Sigma-Aldrich
MOPS, anhydrous, free-flowing, Redi-Dri, ≥99.5%
Sigma-Aldrich
Formamide, ReagentPlus®, ≥99.0% (GC)
Sigma-Aldrich
Formamide, spectrophotometric grade, ≥99%
Sigma-Aldrich
MOPS, Vetec, reagent grade
Sigma-Aldrich
Formamide, Vetec, reagent grade, 98%
Lysine hydrochloride, European Pharmacopoeia (EP) Reference Standard
Supelco
L-Lysine monohydrochloride, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Xylene Cyanol FF, Dye content ≥75 %
Sigma-Aldrich
Bromophenol Blue, ACS reagent
Sigma-Aldrich
Formamide solution, suitable for NMR (reference standard), 90% in DMSO-d6 (99.9 atom % D), NMR tube size 10 mm × 8 in.
Supelco
Formamide solution, suitable for NMR (reference standard), 90% in DMSO-d6 (99.9 atom % D), NMR tube size 5 mm × 8 in.
Supelco
L-Lysine monohydrochloride, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sigma-Aldrich
Ethylenediaminetetraacetic acid disodium salt solution, BioUltra, pH 8.0, ~0.5 M in H2O
Supelco
L-Lysine hydrochloride solution, 100 mM amino acid in 0.1 M HCl, analytical standard
Sigma-Aldrich
L-Lysine monohydrochloride, BioUltra, ≥99.5% (AT)
Sigma-Aldrich
Ethylenediaminetetraacetic acid, BioUltra, ≥99.0% (KT)
Sigma-Aldrich
Ethylenediaminetetraacetic acid, ≥98.0% (KT)
Sigma-Aldrich
Ethylenediaminetetraacetic acid, 99.995% trace metals basis
Supelco
Formamide solution, suitable for NMR (reference standard), 90% in DMSO-d6 (99.9 atom % D), NMR tube size 5 mm × 8 in.
Sigma-Aldrich
L-Lysine monohydrochloride, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
Sigma-Aldrich
Ethylenediaminetetraacetic acid solution, 0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
Ethylenediaminetetraacetic acid, anhydrous, crystalline, BioReagent, suitable for cell culture
Sigma-Aldrich
Xylene Cyanol FF, BioReagent, Molecular Biology