Skip to Content

CN

Total monoamine oxidase (MAO) inhibition by chestnut honey, pollen and propolis.

Total monoamine oxidase (MAO) inhibition by chestnut honey, pollen and propolis.

Journal of enzyme inhibition and medicinal chemistry (2013-10-26)

O Yildiz, F Karahalil, Z Can, H Sahin, S Kolayli

ABSTRACT

Monoamine oxidase (MAO) inhibitors are generally used in the treatment of depressive disorders and some neurodegenerative illnesses, such as Parkinson's disease and Alzheimer's disease. The aim of this preliminary study was to investigate the MAO [MAO (E.C.1.4.3.4)] inhibiting effect of various apitherapeutic products, such as chestnut honey, pollen and propolis. Extracts' MAO inhibition was measured using peroxidase-linked spectrophotometric assay in enzyme isolated from rat liver microsomes, and the values are expressed as the inhibition concentration (IC50) causing 50% inhibition of MAO. The antioxidant activity of the bee products was also determined in terms of total phenolic content (TPC) and ferric reducing/antioxidant power in aquatic extracts. All samples exhibited substantial inhibition of MAO, propolis having the highest. Inhibition was related to samples' TPCs and antioxidant capacities. These results show that bee products possess a sedative effect and may be effective in protecting humans against depression and similar diseases.

MATERIALS

Product Number

Brand

Product Description

Supelco

2,4,6-Tris(2-pyridyl)-s-triazine, for spectrophotometric det. of Fe, ≥99.0% (HPLC)

Supelco

4-Aminoantipyrine, for spectrophotometric det. of H₂O₂ and phenols, ≥98.0%

Sigma-Aldrich

4-Aminoantipyrine, puriss. p.a., reag. Ph. Eur., ≥99%

Sigma-Aldrich

4-Aminoantipyrine, reagent grade

Supelco

2,4,6-Tris(2-pyridyl)-s-triazine, ≥98%

Supelco

4-Aminoantipyrine, analytical standard

Supelco

Phenol solution, certified reference material, 500 μg/mL in methanol

Supelco

Phenol solution, 5000 μg/mL in methanol, certified reference material

Supelco

Phenol, Pharmaceutical Secondary Standard; Certified Reference Material

Sigma-Aldrich

Phenol, puriss., meets analytical specification of Ph. Eur., BP, USP, ≥99.5% (GC), crystalline (detached)

Sigma-Aldrich

Phenol, ACS reagent, ≥99.0%

Sigma-Aldrich

Phenol, ≥99%

Sigma-Aldrich

1,1-Diphenyl-2-picrylhydrazine, 97%

Sigma-Aldrich

Phenol, BioUltra, Molecular Biology, ≥99.5% (GC)

Sigma-Aldrich

Phenol, puriss. p.a., ACS reagent, reag. Ph. Eur., 99.0-100.5%

Sigma-Aldrich

Phenol, BioUltra, Molecular Biology, TE-saturated, ~73% (T)

Supelco

Phenol solution, 100 μg/mL in acetonitrile, PESTANAL^®, analytical standard

Supelco

Phenol, PESTANAL^®, analytical standard

Sigma-Aldrich

Phenol, ≥96.0% (calc. on dry substance, T)

Sigma-Aldrich

Phenol, unstabilized, ReagentPlus^®, ≥99%

Sigma-Aldrich

N-Methyl-N-propargylbenzylamine, 97%

Sigma-Aldrich

Phenol, unstabilized, purified by redistillation, ≥99%

Sigma-Aldrich

Phenol, BioXtra, ≥99.5% (GC)

Sigma-Aldrich

Phenol solution, BioReagent, Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, Molecular Biology

Sigma-Aldrich

Phenol, Molecular Biology

Sigma-Aldrich

Liquified Phenol, ≥89.0%

Sigma-Aldrich

Phenol solution, Saturated with 0.01 M citrate buffer, pH 4.3 ± 0.2, BioReagent, Molecular Biology

Sigma-Aldrich

Phenol, puriss., meets analytical specification of Ph. Eur., BP, USP, 99.5-100.5% (GC)

Sigma-Aldrich

Phenol, natural, 97%, FG

USP

Phenol, United States Pharmacopeia (USP) Reference Standard