Selective Vaporization of Superheated Nanodroplets for Rapid, Sensitive, Acoustic Biosensing.

Selective Vaporization of Superheated Nanodroplets for Rapid, Sensitive, Acoustic Biosensing.

Advanced healthcare materials (2015-06-19)

Rajarshi Chattaraj, Praveena Mohan, Jeremy D Besmer, Andrew P Goodwin

ABSTRACT

Superheated perfluorocarbon nano-droplets exhibit promise as sensitive acoustic biosensors. Aggregation of biotin-decorated lipid-shelled droplets by streptavidin greatly increases the yield of bubbles formed by ultrasound-induced vaporization. Streptavidin is sensed down to 1 × 10(-13) m, with differentiable signal appearing in as little as two minutes, using a scalable assay without washing, processing, or development steps.

MATERIALS

Product Number

Brand

Product Description

S6191

Sigma-Aldrich

Sodium chloride, BioPerformance Certified, ≥99% (titration), suitable for insect cell culture, suitable for plant cell culture

59222C

SAFC

Sodium chloride solution, 5 M

450006

Sigma-Aldrich

Sodium chloride, AnhydroBeads^™, −10 mesh, 99.999% trace metals basis

RDD008

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Trizma^® base, anhydrous, free-flowing, Redi-Dri^™, ≥99.9%

V900483

Sigma-Aldrich

Trizma^® base, Vetec^™, reagent grade, ≥99%

V900058

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Sodium chloride, Vetec^™, reagent grade, 99%

S0817

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93352

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Trizma^® base, ≥99.0% (T)

71386

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Sodium chloride solution, BioUltra, for molecular biology, ~5 M in H₂O

93362

Sigma-Aldrich

Trizma^® base, BioUltra, for molecular biology, ≥99.8% (T)

451940

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Sodium chloride-³⁵Cl, 99 atom % ³⁵Cl

378860

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Sodium chloride, random crystals, optical grade, 99.9% trace metals basis

204439

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Sodium chloride, 99.999% trace metals basis

252859

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Tris(hydroxymethyl)aminomethane, ACS reagent, ≥99.8%

553468

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Tris(tert-butoxy)silanol, 99.999%

T4661

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Trizma^® base, ≥99.9% (titration), crystalline

S1679

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Sodium chloride, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%

T1503

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Trizma^® base, Primary Standard and Buffer, ≥99.9% (titration), crystalline

S6546

Sigma-Aldrich

Sodium chloride solution, 5 M

N0253

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Nitrilotriacetic acid trisodium salt, Sigma Grade, ≥98%

S7653

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Sodium chloride, BioXtra, ≥99.5% (AT)

S5886

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Sodium chloride, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

S3014

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Sodium chloride, for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)

S6150

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93350

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71376

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S5150

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Sodium chloride solution, 5 M in H₂O, BioReagent, for molecular biology, suitable for cell culture

T6066

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T6791

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S8776

Sigma-Aldrich

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