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Merck
CN

H9394

汉克斯平衡盐 溶液

Modified, with sodium bicarbonate, without calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture

别名:

HBSS

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关于此项目

NACRES:
NA.75
UNSPSC Code:
12352207
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Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.011 g/L

shipped in

ambient

storage temp.

room temp

General description

Hanks平衡盐溶液推荐用于悬浮培养或细胞结块时。

Application

Hanks′平衡盐溶液已用于:
  • 作为小鼠皮层切片培养基组分
  • 作为解离缓冲液组分,用于人诱导多能干细胞(hiPSCs)和hiPSC来源心肌细胞(CMs)培养
  • 小鼠肝脏的原位灌注

Biochem/physiol Actions

平衡盐溶液在细胞培养中起多种作用,包括作为冲洗、运输和稀释用液以维持细胞内外的渗透压平衡,供给细胞正常代谢所需的水和大量无机离子。还参与提供缓冲体系,维持培养基的生理pH范围(7.2-7.6),提供细胞代谢所需的主要能量。


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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

Trypsin is commonly used for dissociating adherent cells from surfaces. A wide variety of trypsin solutions are available to meet your specific cell line requirements.

StableCell™ Trypsin solutions are designed to perform cell detachment as standard trypsin solutions do, without the need to aliquot, freeze, and thaw. This saves significant time before passaging.

胰蛋白酶可用于从培养表面去除贴壁细胞。从培养基底物除去细胞的最常用方法是用胰蛋白酶或胰蛋白酶/EDTA溶液处理。

查看所有协议

Annemiek A van Berkel et al.
Journal of neurochemistry, 157(3), 450-466 (2020-12-02)
Loss of the exocytic Sec1/MUNC18 protein MUNC18-1 or its target-SNARE partners SNAP25 and syntaxin-1 results in rapid, cell-autonomous and unexplained neurodegeneration, which is independent of their known role in synaptic vesicle exocytosis. cis-Golgi abnormalities are the earliest cellular phenotypes before
Sarah Pedretti et al.
PloS one, 14(6), e0218432-e0218432 (2019-06-21)
High density lipoprotein (HDL) protects against myocardial infarction via mechanisms that remain unclear. STAT3 (signal transducer and activator of transcription 3) plays a key role in HDL-induced cardioprotection. In the heart, microRNAs (miRNAs) are involved in ischemia reperfusion injury. We
Margret Bessadottir et al.
PloS one, 7(12), e51296-e51296 (2012-12-12)
The lichen compound usnic acid (UA) is a lipophilic weak acid that acts as a proton shuttle and causes loss of mitochondrial inner membrane potential. In the current study we show that UA treatment induced the formation of autophagosomes in



全球贸易项目编号

货号GTIN
H9394-6X1L04061833812624
H9394-6X500ML04061833812631
H9394-1L04061833812501
H9394-24X500ML04061833812549
H9394-100ML04061833812495
H9394-500ML04061833812563